Speciation

Adaptive Landscape

An example of adaptive landscape in bacteria will be outlined in lecture.

Species concepts

Biological species concept -- "Species are groups of actually or potentially interbreeding populations, which are reproductively isolated from other such groups." Mayr 1942

Hybrid sterility definitely separates two groups as species though it is not the sole criterion

Reproductive isolation could however be due to behavioral or morphological blocks to mating (i.e., hybrids are possible but unlikely given such blocks).

Barriers are not merely geographic, but have a biological origin and arise during the process of speciation.

Isolating mechanisms:

A) Premating isolating mechanisms -- prevent union of gametes -> zygote

  1. mates do not meet (seasonal or habitat isolation)
  2. mates meet but do not mate (ethological or behavioral isolation)
  3. mates meet but no sperm transfer (mechanical isolation)

B) Post-mating isolating mechanisms -- varying degrees of hybrid sterility

  1. sperm transfered but dies before fertilization
  2. zygote dies
  3. zygote produces an F1 adult that has reduced viability (survival)
  4. hybrid is viable, but partially or completely sterile (fecundity) or the F2 is deficient.

And, we can have both A) premating and B) post-mating simultaneously

The biological species concept begins to break down in areas or at times of speciation or incipient speciation (semispecies). For example at hybrid zones, the somewhat differentiated populations are in the process of speciation.

Genetic similarity drops as we change from the different levels of differentiation (c.f., Wright's population subdivision). (overhead)

Populations - Subspecies - Semispecies - Sibling species - Non-sibling species -

Species -- NO GENE FLOW

Clearly genetic differentiation is occuring but it is not enough to define a species. It can vary from organism to organism and from mode of speciation to the next.

Migration is the movement of animals between what are referred to as populations of interbreeding animals. Rates of migration are usually expressed as the proportion of individuals in a population that disperse from one population to the next each generation. If migration between the region of contact between the two semispecies and the rest of the semispecies ranges is too high, then the two semispecies will become homogenized into one species even if such interbreeding results in lower fitness because of hybrid unfitness (Endler, 1977). If migration is low enough, then the two pure semispecies will eventually become differentiated into two species owing to the evolution of isolating mechanisms. Evolution of such isolating mechanisms is contingent upon the degree of genetic similarity between the two semispecies.

Figure 5.1. Hypothetical hybrid "unfitness" resulting from an incompatibility between genomes of the two parent species.

Large differences in genetic similarity between semispecies are more likely to produce unfit hybrids than small differences in similarity. If the two semispecies have many genetic loci that differ in the kind of allele that they possess, then those alleles might not work very well together, and more importantly the way two different genetic loci work together may begin to breakdown. This is referred to as genome-wide genetic interactions. The correct operation of one genetic locus (lets say an enzyme) may require a particular form of the enzyme that is produced by a different locus (e.g., epistasis) (Whitlock et al 1993). These enzymes are most likely to be compatible in their function if they come from the same species. If they are from different species, they may not integrate well with other loci in the organism and the hybrid individual may die. Imagine such genetic interactions occurring at all possible loci (e.g., 10,000 loci in Drosophila or perhaps 100,000 loci in humans). The probability of a hybrid being more unfit has to do with how many loci in each semispecies are homozygous for different alleles. Hybrid breakdown occurs when many of these loci are fixed for different alleles in the two subspecies and the production of offspring by the hybrids is not possible because and F1 F1 produces too many incompatible gene by gene combinations.



MODES OF SPECIATION -- CLASSIFIED BY GEOGRAPHY.

  1. Allopatric Speciation -- speciation occurs in geographic isolation
  2. Parapatric Speciation -- speciation in adjacent populations with gene flow
  3. Allo-Parapatric Speciation -- populations initially separated (allo-) but then secondarily come into contact with subsequent parapatric speciation
  4. Sympatric Speciation -- speciation within a panmictic population.

Modes of Speciation Classified by Geography

To understand the origin of species differences it is necessary to consider how genetic differences arise between species. Geographic subdivision decreases gene flow. Depending on the population size, genetic drift can play a major role in promoting genetic differentiation. If population size remains low for a long period of time it is possible for genetic differences to accumulate between geographic areas by the random process of genetic drift. Selection can promote speciation because genetic changes can rapidly accumulate in one area where selection favors certain behaviors, physiology, or morphology relative to another area where alternative alleles are favored by natural selection. Finally, the amount of gene flow which is usually described in terms of the migration rate is a key parameter that influences the rapidity of speciation. By lowering gene flow, geographic subdivision promotes the process of speciation (Endler, 1977). Ernst Mayr (1942) has argued that some form of geographical subdivision is required for speciation.

Figure Legend. Models of speciation based on geographical subdivision. Models of speciation relate to the degree of geographical subdivision for Allopatric --> Parapatric --> Sympatric which ranges extreme to none. Founder effect speciation is an extreme form of subdivision in species formation takes place in a small isolated population.


Most models of speciation relate to the degree of geographical subdivision:

  1. Allopatric Speciation -- speciation occurs in geographic isolation,
  2. Founder Effect Speciation -- a special kind of allopatric speciation in a small isolated population on the edge of a species range
  3. Parapatric Speciation -- speciation in adjacent populations with gene flow,
  4. Allo-Parapatric Speciation -- populations initially separated (allo-) but then secondarily come into contact with subsequent parapatric speciation,
  5. Sympatric Speciation -- speciation within a panmictic population.

Founder Effect or Peripatric Speciation -- speciation occurs in an isolated population.These relate to the degree of geographical subdivision which ranges from:

Extreme <--> None

for the modes: Allopatric -- Allo-parapatric -- Parapatric -- Sympatric

Patterns -- Variation within a species is at the heart of the matter.

CLINES:

  1. Presence no cline. -- no contact, species have no gene flow due to isolation
  2. Shape of the Cline. To what degree is the variation clinal (e.g., at a hybrid zone). Clines can be gradual along a transect or very abrupt (within a few 100 m whereas a species is spread over 1000 km).
  3. Origin of the cline or hybrid zone. Is it due to secondary contact with subsequent weak gene flow across the edge. Evidence for secondary contact can include concordant variation in many characters on either side of the cline. OR Is the cline maintained by persistent selection? In this case the cline is primary.
  4. No cline but sympatric -- speciation in situ due to temporal, ecological, (or cytological) segregation. Clines are not an issue.



Figure 5.3. Hypothetical change in frequency of allele frequency across the range of two species under allopatric, parapatric, and sympatric models of speciation.

  1.  

Big Question: Can we distinguish between primary and secondary contact?


ALLOPATRIC SPECIATION

By isolation of a colony (e.g., island and mainland)

By division of range by extrinsic barrier or extinction of populations

By geographical distance.

Ring Species are a peculiar form of speciation that takes place when the center of a species range is effectively unoccupied and thus there is no gene flow. Adjacent races will interbreed, however the races at the termini are so divergent, that there is no interbreeding (exist in sympatry).

Fig. 3, p 225 (of Futuyma) Thamnophis (garter snakes in California) (overhead)

Fig. 4-4, p. 65 (of Price) Sea Gulls and the Arctic Ocean

Hybrid zones -- Tend to show underdominance in fitness, that is heterozygotes (hybrids) have lower fitness, and this is taken as evidence of the hybrid zone being due to secondary contact.

Introgressive hybridization -- If there is any viability of the hybrids, alleles may leak across and introgress across the zone. Some alleles may spread quite far across the zone whereas others will be quite abrupt and coincide with the hybrid zone.


PARAPATRIC SPECIATION

Clines -- may arise from environmental difference and selection.

Consider a simple population genetic model. Selection along a string of demes.

(From Figure 6, p. 162, chap VI, of Futuyma). (overhead).

In this model the form of the cline is primarily determined by selection.

A second model by Slatkin (1973) -- The width of the cline arising from selection is given by s/where s is the standard deviation of dispersal distance, and s is selection against the wrong allele.

Why should the clines in a number of loci be concordant?

linkage - - alleles are "dragged" along (requires many such linkage groups)

epistasis - - fitness at one locus depends on genotype subject to clinal selection

If the cline is primary (i.e., not from secondary contact), then there is the possibility that parapatric speciation was responsible for the divergence.

However, parapatric speciation is greatly debated.


SYMPATRIC SPECIATION

One form is widely accepted -- the instantaneous mode in which changes in ploidy lead to instantaneous reproductive isolation (e.g., in plants). The examples from chapter 22 are used in lecture.

Most other theories involving a gradual change in sympatry are controversial.

One class of models maintains that there arise multiple-niche polymorphisms (heterozygotes inferior). Assortative mating among homozygotes is a key component of this theory. This minimally requires two loci to evolve simulatneously, and thus we establish strong linkage disequilibrium for the niche polymorphism loci and the assortative mating loci. More loci makes it unlikely.

 

Sympatric Speciation

The evolution of such divergence, in the face of gene flow is a tremendous challenge for the evolution of species by the process of sympatric speciation. In a classic population genetic analysis, Felsenstein (1981) showed that the process of sympatric speciation is a fairly unlikely event. He modeled this process in terms the evolution of two species attributes: 1) the phenotypic attributes that give rise to hybrid unfitness, and 2) the genes that give rise to mate discrimination. Generally, the genes for fitness, are not expected to be linked (either by pleiotropy or by physical linkage) to the genes that give rise to mate discrimination. This key fact places a tremendous genetic constraint on speciation by the process of reinforcement. Every generation segregation of chromosomes will lead to parental species that lack the correct discrimination alleles. Even if the genes fitness and mate discrimination are linked on the same chromosome, recombination during meiosis is a powerful enough force to dissolve away such associations and eliminate the possibility of sympatric speciation.- from the time of the modern synthesis up until very recently, the importance of sympatric speciation was greatly downplayed.

- the main reason for this was Ernst Mayr.

- Mayr championed the allopatric mode of speciation, and there is little doubt that Mayr is correct in recognizing the predominance of allopatric speciation in nature.

- however, Mayr was very adamant about allowing that even a small portion of species may be speciating by the sympatric mechanism.

- one of the major reasons why evolutionary biologists now consider the possibility that sympatric speciation is a more common mode of speciation than originally thought has been a result of the work of Guy Bush.

- Guy Bush was a recently graduated Ph.D. student at Harvard in 1966, when he gave his first paper at a scientific conference.

- he was nervous and had a right to be nervous.

- his Ph.D. work had pointed to the possibility that a new species of Rhagoletis fruit fly was forming right beside its parent population by the process of sympatric speciation.

- situated in the audience listening to his talk were Dobzhansky, Mayr and other leading figures of the modern synthesis.

- according to the story, after he finished his talk, there were a few moments of deafening silence from the audience.

- then Dobzhansky spoke - he said "That's very interesting, but I don't believe it. Sympatric speciation is like the measles. Everybody gets it, but they soon get over it."

- Bush never quite got over it - he pursued the Rhagoletis story for the next 20 years, making it one of the best known cases of sympatric speciation known to date.

Simple model:

2 loci involved H locus = "host selection" locus

S locus = "larval survival" locus

- suppose a species of fruitfly exists that mates and lays eggs on apple trees.

suppose: H1 = prefer apple

S1 = survive on apple

- the species would be H1H1S1S1

- suppose a new allele emerged, H2 that caused a shift in host tree preference from apple to cherry.

- if survival of the larvae on cherry was low, a new allele at the S locus, S2, might occur that greatly increases survival on cherry but does not survive on apple.

H2 = prefer cherry

S2 = survive on cherry

- selection would thus favor the H2H2S2S2 genotype on cherry, which could lead to a new species with different host preference.

- the crucial factor is what heterozygotes tend to do.

- however, these fruitflies have a strong tendency to remain and oviposit on the tree in which they emerged.

- this might explain in this example why speciation might occur so readily.


GENETIC THEORIES OF SPECIATION -- Templeton's Cohesion Species Concept this is a completely different classification scheme from the Geographic modes of speciation. (Genes are the focus not geography).

  1. Runaway process and sexual selection -- divergence in mate preference in isolation.
  2. Gradual divergence -- Force of selection changes in two spatially separated populations.
  3. Genetic revolution -- Multiple peaks are present but genetic drift is required to move a population against the force of selection (adaptive valley) to the knew adaptive peak.
  4. Chromosomal differentiation -- peak shift Ploidy changes (previously discussed) well established, as well as small changes like fusions and fission that only have small effect on fitness (c.f., translocations with large decrements to fitness) for the following reasons:

Problems with peak shift

  1. Consider the simple one allele case where heterozygotes are at a disadvantage
  2. To move from high frequency of one allele (or chromosomal type) to high frequency of another allele (or chromosomal type) the number of heterozygotes must increase (which are deleterious)
  3. Reproductive isolation is enhanced by large decrements in heterozygote fitness.
  4. Selection is strong so traversing the low saddle (against fitness) is only possible in small populations

Given saddle, peak -- the probability of peak shift is prop. to Ne. If Ne is small, the probability of peak shift is large, if Ne is large, the probability of peak shift is trivial.


FOUNDER EFFECT (PERIPATRIC SPECIATION) Mayr's Theory

  1. On the continent or in the large population Genetic homeostasis and gene flow opposes divergence.
  2. Genetic change is rapid and pervasive in small populations with no gene flow
  3. Sampling effects lead to dramatic differences in gene frequency
  4. The genetic environment is altered (epistasis -- interactions among genes)
  5. This alteration of epistasis leads to massive genetic change (lots of fitness effects have changed).

Arguments against founder effect: Sampling effects are only important if inbreeding kicks in (the population remains bottlenecked for lots of generations.)

Arguments in favor:

  1. experiments such as drosophila (bottle experiments)
  2. biogeographic patterns (Drosophilidae of Hawaii, p. 304, fig 13) (overhead)