Cholesterol oxidase modelled in contact with cellular membrane and cholesterol
Sampson NS, Vrielink A. Cholesterol Oxidases: A Study of Nature's Approach to Protein Design.
Acc Chem Res. 2003 Sep 16;36(9):713-722 HTML/PDF
Vrielink, A., Sampson, N.S.
Sub-Ångstrom Resolution Enzyme X-ray Structures: Is Seeing Believing?
Curr Opin Struct Biol. 2003 Dec;13(6):709-15. HTML/PDF
Lario PI, Vrielink A.
Atomic Resolution Density Maps Of the Backbone Carbonyl Groups Reveal Secondary Structure Dependent Differences in Electronic Distribution. J Am Chem Soc. 2003 Oct 22;125(42):12787-94. HTML/PDF
Manjasetty BA, Powlowski J, Vrielink A.
Crystal structure of a bifunctional aldolase-dehydrogenase: sequestering a reactive and volatile intermediate. Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):6992-7. Epub 2003 May 22: HTML/PDF
Lario PI, Sampson N, Vrielink A. (2003)
Sub-atomic Resolution Crystal Structure of Cholesterol Oxidase: What Atomic Resolution Crystallography Reveals about Enzyme Mechanism and the Role of the FAD Cofactor in Redox Activity. J Mol Biol 326(5):1635-50 HTML/PDF
Gao YS, Vrielink A, MacKenzie R, Sztul E. (2002)
A novel type of regulation of the vimentin intermediate filament cytoskeleton by a Golgi protein. Eur J Cell Biol 81(7):391-401 PDF
Burns CS, Aronoff-Spencer E, Dunham CM, Lario P, Avdievich NI, Antholine WE, Olmstead MM, Vrielink A, Gerfen GJ, Peisach J, Scott WG, Millhauser GL. (2002)
Molecular features of the copper binding sites in the octarepeat domain of the prion protein. Biochemistry 41(12):3991-4001 HTML/PDF
Ye, Y. Sampson, N.S., Vrielink, A., & Lario, P.I. (2001).
The presence of a hydrogen bond between asparagine 485 and the pi system of FAD modulates the redox potential in the reaction catalyzed by cholesterol oxidase. Biochemistry 40, 13779-13787. HTML/PDF
Geyer, A., Fitzpatrick, T.B., Pawelek,, P., Kitzing, K., Vrielink,
A., Ghisla, S. & Macheroux, P. (2001).
Structure and characterization of the glycan moiety of
L-amino acid oxidase from the malayan pit viper Calloselasma
rhodostoma. European Journal of Biochemistry,
268, 4044-53. Abstract
Lario, P.I., Bobechko, B., Bateman, K., Vrielink, A. &
Huang, Z. (2001).
Purification and Characterization of the Human PDE4A Catalytic Domain
(PDE4A330–723) Expressed in Sf9 Cells.
Archives of Biochemistry and Biophysics 394, 54-60. PDF
Coulombe, R., Yue, K.Q., Ghisla, S. & Vrielink, A. (2001).
Oxygen access to the active site of cholesterol oxidase
through a narrow channel is gated by an Arg-Glu pair. Journal
of Biological Chemistry 276, 30435-30441. HTML/PDF
Manjasetty, B.A. Croteau, N. Powlowski, J & Vrielink, A.
(2001).
Crystallization and preliminary x-ray analysis of dmpFG-encoded
4-hydroxy-2-ketovalerate aldolase-aldehyde dehydrogenase (acylating)
from Pseudomonas sp. strain CF600. Acta Crystallographica
D 57, 582-585. HTML/PDF
Ahvazi, B., Zhang, L., Ahvazi, B., Szittner, R., Vrielink,
A., & Meighen, E (2000).
A histidine residue in the catalytic mechanism distinguishes
Vibrio harveyi aldehyde dehydrogenase from other members
of the aldehyde dehydrogenase superfamily. Biochemistry.
39,14409-14418. HTML/PDF
Pawelek, P., Cheah, J., Coulombe, R., Macheroux, P., Ghisla,
S. & Vrielink, A. (2000).
The structure of L-amino acid oxidase reveals the substrate
trajectory into an enantiomerically conserved active site. EMBO
Journal 19, 4204 - 4215. HTML/PDF
Ahvazi, B., Coulombe, R. Delarge, M., Zhang, L., Vedadi, M.,
Meighen, E. & Vrielink, A. (2000)
Crystal structure of the NADP+ dependant aldehyde dehydrogenase
from Vibrio harveyi. Structural implications for cofactor specificity
and affinity. Biochemical Journal 349, 853 - 861. HTML/PDF
Kohls, D., Sulea, T., Purisima, E., MacKenzie, R. & Vrielink,
A. (2000).
The crystal structure of the formiminotransferase domain
of formiminotransferase-Cyclodeaminase: Implications for substrate channeling
in a bifunctional enzyme. Structure 8, 35-46.
HTML/PDF
Zhang, L. Ahvazi, B. Szittner, R., Vrielink, A. & Meighen,
E. (1999).
Change of nucleotide specificity and enhancement of catalytic
efficiency in single point mutants of Vibrio harveyi aldehyde dehydrogenase.
Biochemistry 38, 11440-11447. HTML/PDF
Yue, K.Q., Kass, I.J., Sampson, N.S. & Vrielink, A. (1999)
Crystal Structure Determination of Cholesterol Oxidase
from Streptomyces and Characterization of Key Active Site
Mutants. Biochemistry, 38, 4277-4286 HTML/PDF