EDUCATION

1975            B.S., Microbiology, University of Minnesota.  Undergrad research with Conrad Firling and Robert McKinnel

1982            M.S., Biology, California State University, Northridge with Steven B. Oppenheimer and Phillip Sheeler

1986            Ph.D., Molecular, Cell and Developmental Biology, University of Colorado, Boulder with J. Richard McIntosh.  Tubulin Dynamics in Mitotic and Interphase Cells.

1990            Postdoctoral Fellow, Dept. of Biology, with Elizabeth Raff.  Genetics of the Cytoskeleton and Microtubule Motors.

PROFESSIONAL APPOINTMENTS

1990-97      Assistant Professor, Dept. of Biology, Indiana University

1990-97      Fellow of The Indiana Molecular Biology Institute

1997-03      Associate Professor, Dept. of Biology, , Indiana University

1997-07      Senior Fellow of The Indiana Molecular Biology Institute

2003-07      Professor, Dept. of Biology, Indiana University

2007-          Professor, Dept. of MCD Biology, University of California Santa Cruz

FELLOWSHIPS AND AWARDS

1982-84      NIH Predoctoral Trainee

1984            University of Colorado Foundation Fund Research Award

1986            University of Colorado Outstanding Graduate Student Award

1986-89      American Cancer Society Postdoctoral Fellowship

1994-99      American Heart Association Established Investigatorship

2003            Indiana University Trustees Award for Teaching Excellence

2004            Wallace Franklin Medal

PUBLICATIONS

1.      Meyer, J.T., P.M. Thompson, R. Behringer, R.C. Steiner, W.M. Saxton and S.B. Oppenheimer.  1983.  Protease activity associated with loss of adhesiveness in mouse teratocarcinoma.  Exp. Cell Res. 143:63-70.

2.      Leslie, R.J., W.M. Saxton, T.J. Mitchison, B. Neighbors, E.D. Salmon and J.R. McIntosh.  1984.  Assembly properties of fluorescein labeled tubulin in vitro before and after fluroescence belaching.  J. Cell Biol. 99:2146-2156.

3.      Salmon, E.D., W.M. Saxton, R.J. Leslie, M.L. Karow, and J.R. McIntosh.  1984.  Diffusion coefficient of fluorescein-labeled tubulin in the cytoplasm of embryonic cells of a sea urchin:  Measurement by video image processing of fluorescence redistribution after photobleaching.  J. Cell Biol. 99:2157-2164.

4.      Salmon, E.D., R.J. Leslie, W.M. Saxton, M.L. Karow and J.R. McIntosh.  1984.  Spindle microtubule dynamics in sea urchin embryos.  Analysis using a fluorescein-labeled tubulin and measurements of fluorescence redistribution after laser photobleaching.  J. Cell Biol. 99:2165-2174.

5.      Saxton, W.M., D.L. Stemple, R.J. Leslie, E.D. Salmon and J.R. McIntosh.  1984.  Tubulin dynamics in cultured mammalian cells.  J. Cell Biol. 99:2175-2186.

6.      McIntosh, J.R., W.M. Saxton, D.L. Stemple, R.J. Leslie, and M.J. Welsh.  1985.  Dynamics of tubulin and calmodulin in the mammalian mitotic spindle.  Ann. NY Acad. Sci. 466:566-579.

7.      Saxton, W.M. and J.R. McIntosh.  1987.  Interzone microtubule behavior in late anaphase and telophase spindles.  J. Cell Biol. 105:875-886.

8.      Saxton, W.M., M.E. Porter, S.A. Cohn, J.M. Scholey, E.C. Raff and J.R. McIntosh.  1988.  Drosophila kinesin:  Characterization of microtubule motility and ATPase.  Proc. Natl. Acad. Sci. USA 85:1109-1113.

9.      Yang, J., W.M. Saxton and L.S.B. Goldstein.  1988.  Isolation and characterization of the gene encoding the heavy chain of Drosophila kinesin.  Proc. Natl. Acad. Sci. USA 85:1864-1868.

10.  Olmsted, J.B., D.L. Stemple, W.M. Saxton, B.W. Neighbors and J.R. McIntosh.  1989.  Cell cycle-dependent changes in the dynamics of MAP 2 and MAP 4 in cultured cells.  J. Cell Biol. 109:211-223.

11.  Yang, J., W.M. Saxton, M. deCuevas, E.C. Raff and L.S.B. Goldstein.  1990.  Evidence that the head of kinesin is sufficient for force generation and motility in vitro.  Science 249:42-47.

12.  Saxton, W.M., J. Hicks, L.S.B. Goldstein and E.C. Raff.  1991.  Kinesin heavy chain is essential for viability and neuromuscular functions in Drosophila but mutants show no defects in mitosis.  Cell 64:1093-1102.

13.  Gho, M., K. McDonald, B. Ganetzky, and W.M. Saxton.  1992.  Effects of kinesin mutations on neuronal functions.  Science 258:313-316.

14.  Cohn, S., W.M. Saxton, J. Lye and J. Scholey.  1993.  Analyzing microtubule motors in real time.  Methods in Cell Biology 39:75-88.

15.  Saxton, W.M.  1994.  Isolation and analysis of microtubule motor proteins.  Methods in Cell Biology 44:279-288.

16.  Cole, D. G., K.B. Sheehan, W.M. Saxton, and J.M. Scholey. 1994. A"slow" heterotetrameric kinesin-related motor protein purified from Drosophila embryos.  J. Biol. Chem. 269:22913-22916.

17.  Hurd, D.D., M. Stern, and W.M. Saxton.  1996.  Mutation of the axonal transport motor kinesin enhances paralytic and suppresses Shaker in Drosophila.  Genetics 142:195-204.

18.  Kashina, A., R. Baskin, D. Cole, W.M. Saxton, and J. Scholey. 1996. A bipolar kinesin.  Nature 379: 270-272.

19.  Hurd, D.D. and W.M. Saxton. 1996.  Disruption of fast axonal transport by kinesin mutations causes motor neuron disease phenotypes in Drosophila.  Genetics 144:1075-1085.

20.  Kashina, A.S.,  J.M. Scholey, J.S. Leszyk, and W.M. Saxton. 1996.  KLP61F/KRP130 is a bipolar mitotic motor.  Nature 384:225.

21.  Powers, J., O. Bossinger, D.J. Rose, S. Strome, and W.M. Saxton. 1998.  A nematode kinesin required for cleavage furrow advancement.  Current Biology 8:1133-1136.

22.  Saxton, W.M..  A special delivery service.  1999.  Current Biology 9:293-295.

23.  Martin, M.E., D.D. Hurd and W.M. Saxton.  1999.  Kinesins in the Nervous System.  Cell. Mol. Life Sci. 56:200-216.

24.  Brendza, K.M., D.J. Rose, S.P. Gilbert, and W.M. Saxton.  1999.  Lethal kinesin mutations reveal amino acids important for ATPase activation and structural coupling.  J. Biol. Chem. 274:31506-31514.

25.  Martin, M.E., S.J. Iyadurai, A. Gassman, J. G. Gindhart, Jr., T.S. Hays, and W.M. Saxton.  1999.  Cytoplasmic Dynein, the Dynactin Complex, and Kinesin Are Interdependent and Essential for Fast Axonal Transport.  Mol. Biol. Cell 10:3717-3728.

26.  Brendza, R.P., K.B. Sheehan, F.R. Turner, and W.M. Saxton.  2000.  Clonal Tests of Conventional Kinesin Function During Cell Proliferation and Differentiation.  Mol. Biol. Cell 11:1329-1343.

27.  Brendza, K.M., C. A. Sontag, W.M. Saxton, and S.P. Gilbert.  2000.  A kinesin mutation Uncouples motor domains and desensitizes the gamma-phosphate sensor.  Journal Biol. Chem. 275:22187-22195.

28.  Brendza, R.P., L.R. Serbus, J.B. Duffy, and W.M. Saxton.  2000.  A Function for Kinesin I in the Posterior Transport of oskar mRNA and Staufen Protein.  Science  289: 2120-2122

29.  Strome, S., J. Powers, M. Dunn, K. Reese, G. Seydoux, and W. Saxton.  2001.  Spindle Dynamics and the Role of g-tubulin in Early C. elegans Embryos.  Mol. Biol. Cell. 12:1751-1764.

30.  Yager, J., S. Richards, D.S. Hekmat-Scafe, D.D. Hurd, V. Sundaresan, D.R. Caprette, W.M. Saxton, J.R. Carlson, and M. Stern.  2001.  Control of Drosophila perineurial glial growth by interacting neurotransmitter-mediated signaling pathways.  PNAS 98:10445-10450.

31.  Saxton, W.M..  2001.  Microtubules, motors, and mRNA localization mechanisms:  watching fluorescent messages move. Cell 107: 707-710.

32.  Brendza, R.P., L.R. Serbus, W.M. Saxton, and J.B. Duffy.  2002.  Posterior localization of dynein and dorsal-ventral axis formation depend on kinesin in Drosophila oocytes. Curr. Biol. 12:1541-1545.

33.  Segbert, C., Barkus, R., Powers, J., Strome, S., Saxton, W. M., Bossinger, O.  2003.  KLP-18, a Klp2 kinesin, is required for assembly of acentrosomal meiotic spindles in Caenorhabditis elegans.  Mol. Biol. Cell 14:1458-1469.

34.  Li, S.,  C. M. Armstrong, N. Bertin, H. Ge1, S. Milstein, M. Boxem, P.O. Vidalain, J. Han, A. Chesneau1, T. Hao, D.S. Goldberg, N. Li, M. Martinez, J.-F. Rual1, P. Lamesch, L. Xu, M. Tewari, S.L. Wong, L.V. Zhang, G.F. Berriz, L. Jacotot, P. Vaglio, J. Reboul, T. Hirozane-Kishikawa, Q. Li, H.W. Gabel, A. Elewa, B. Baumgartner, D.J. Rose, H. Yu, S. Bosak, R. Sequerra, A. Fraser, S.E. Mango, W.M. Saxton, S. Strome, S. Heuvel, F.Piano, J. Vandenhaute, C. Sardet, M. Gerstein, L. Doucette-Stamm, K.C. Gunsalus, J.W. Harper, M.E. Cusick, F.P. Roth, D.E. Hill, M. Vidal.  2004  A Map of the Interactome Network of the Metazoan C. elegans.  Science 303: 540-543.

35.  Klumpp, L.M., K.M. Brendza, J.E. Gatial, A. Hoenger, W.M. Saxton, and S.P. Gilbert. 2004.  Microtubule-kinesin interface mutants reveal site critical for communication.  Biochemistry 43:2792-2803.

36.  Powers, J., D.J. Rose, A. Saunders, S. Dunkelbarger, S. Strome, and W.M. Saxton.  2004.  Loss of KLP-19 polar ejection force causes misorientation and missegregation of holocentric chromosomes.  J. Cell Biol. 166: 991-1001.

37.  Lawrence, C.J., R.K. Dawe, KR. Christie, D.W. Cleveland, S.C. Dawson, S.A. Endow, L.S.B. Goldstein, H.V. Goodson, N. Hirokawa, J. Howard, R.L. Malmberg, J.R. McIntosh, T.J. Mitchison, A.S.N. Reddy, W.M. Saxton, M. Schliwa, J.M. Scholey, R.D. Vale, C.E. Walczak, and L. Wordeman.  2004. A Standardized Kinesin Nomenclature.  J. Cell Biol. 167: 19-22

38.  Schmidt, D., D.J. Rose, W.M. Saxton, and S. Strome.  2005.  Functional Analysis of Cytoplasmic Dynein Heavy Chain in C. elegans With Fast-Acting Temperature-Sensitive Mutations.  Mol. Biol. Cell 16:1200-1212.

39.  Martin, M.A., S.M. Ahern-Djamali, F.M. Hoffmann, and W.M. Saxton.  2005.  Abl tyrosine kinase and its substrate Ena/VASP have functional interactions with kinesin-1.  Mol. Biol. Cell 16:4225-4230.

40.  Serbus, L.R., B.J. Cha, W. Theurkauf, and W.M. Saxton.  2005.  Dynein and the actin cytoskeleton control kinesin-driven cytoplasmic streaming in Drosophila oocytes.  Development 132:3743-3752.

41.  Hollenbeck, P.J. and W.M. Saxton.  2005.  The axonal transport of mitochondria.  J. Cell Sci. 18:5411-5419.

42.  Horiuchi, D.,  R.V. Barkus, A.D. Pilling, A. Gassman, and W.M. Saxton.  2005.  APLIP1, a Kinesin-Binding JNK Scaffold Protein, Influences Bidirectional Transport of Vesicles and Retrograde Transport of Mitochondria in Drosophila Axons. Curr. Biol. 15:2137-2141.

43.  Pilling, A., D Horiuchi, C.M. Lively and W. M. Saxton.  2006.  Kinesin-1 and dynein are the primary motors for fast transport of mitochondria in Drosophila motor axons.  Mol. Biol. Cell 17:2057-2068.

44.  Saunders, A.M., J. Powers, S. Strome, and W.M. Saxton. 2007.  Anaphase spindle elongation:  A molecular motor acts as a brake.  Curr. Biol. 17:r453-455.

45.  Horiuchi, D., C.A. Collins,, R.V. Barkus, P. Bhat, A. DiAntonio, and W.M. Saxton. 2007  Control of a kinesin-cargo linkage mechanism by JNK pathway kinases. Curr. Biol. 17:1313-1317.

46.  Barkus, R.V., O. Klyachko, B.J. Dickson, and W.M. Saxton. 2008.  Identification of an axonal kinesin-3 motor for fast anterograde vesicle transport that activates retrograde transport of neuropeptides.  Mol. Biol. Cell 19:274-283.

GRANTS

1991                Indiana Biomedical Research Support Grant,  $10,000 (D).

1991-96           NIH R01 GM46295:  Mechanisms and Functions of Subcellular motility, $798,792 (D+I).

1994                Co-Investigator, NIH Equipment Grant for transmission electron microscope,  $100,000 (D).

1994-99           American Heart Association Established Investigatorship:  Functions and Mechanisms of Intracellular Motility,  $336,055 (D).

1995                Co-Investigator, I. U. Shared Equipment Grant,  $100,000 (D).

1995-96           Walther Cancer Institute Postdoc. Fellowship (M. Martin),  $20,000 (D).

1995-98           American Heart Association, Predoct Fellowship (K. Stupka),  $48,000 (D).

1996-97           American Heart Association, Postdoc Fellowship (M. Martin), $30,000 (D).

1996-00           NIH R01 GM46295.  Mechanisms and Functions of Subcellular motility, $931,650 (D+I).

1997                NIH-Shared Instrumentation Grant for a Scanning Electron Microscope.  $194,000 (D).

1997-99           NIH Postdoc. Fellowship  (M. Martin) $45,000 (D).

1999-00           Walther Cancer Institute Postdoc. Fellowship (M. Martin),  $20,000 (D).

1999-04           NIH GM58811. Cytoplasmic Motility In Early Development. $886,992 (D+I)

2000-06           NIH R01 GM46295.  Mechanisms and Functions of Subcellular Motility.  $1,134,616 (D+I)

2001-03           INGEN (54-246-02), Bloomington INGEN Light Microscopy Core Facilities.  $334,000 (D)

2001-03           American Heart Association, Predoctoral Fellowship (A. Pilling).  $47,000 (D).

2004-05           NIH--- Funding of Concept Development Award for:  The Biology of Mitotic Motors--A Nanomedicine Consortium (Co-PI with Susan Gilbert and 5 others).  $50,000 (D)

2004-07           American Heart Association Predoctoral Fellowship (Dai Horiuchi).  $75,000 (D)

2004-06           Amer. Heart Assoc. Predoctoral Fellowship (Rosemarie Barkus).  $49,000 (D)

2005-06           MetaCYT Award for Mechanisms of Chromosome and Spindle Pole Movements in Mitosis.  $67,532 (D)

2006                IU COAS FRSP Award for An ultra-sensitive, high-speed spinning disk microscope.  $104,856 (D)

2006-07           MetaCYT Award for Mechanisms of Chromosome and Spindle Pole Movements in Mitosis.  $67,250 (D)

2006-10           NIH R01 GM46295.  Mechanisms and Functions of Subcellular Motility.  $1,242,076 (D+I)